Review

human il 33 duoset elisa kit (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems human il 33 duoset elisa kit

A CellChat analysis of human lung snRNA-seq data. B Venn of 47 shared DEGs from four datasets ( GSE159354 , GSE132771 , GSE128033 , GSE122960 ). C Heatmap of 47-gene expression across cell types. D Correlation analysis (Pearson Correlation) of expression of IGFBP5, IL33, MGP, SPRY1, ACKR1 and mechanical stress scores in ECs. E Correlation analysis (Pearson Correlation) of expression of PIEZO1 and IGFBP5, IL33, MGP, SPRY1, ACKR1 in ECs. F Human snRNA-seq UMAP and violin of IL33 distribution and subpopulations. G Bar chart showed expression of IL33 (mean ± SEM) in IPF (n = 7966 cells) vs control (n = 4174 cells). P value was calculated using the two-tailed unpaired t-test. H Violin of IL33 in PIEZO1 - vs PIEZO1 + ECs. I Bulk-RNA-seq of saline and BLM (7 days and 14 days) induced mice (n = 3 per group). Gene expression levels were Z-score normalized. P value was calculated using the two-tailed unpaired t-test. J qPCR of Il33 in BLM mouse lung ECs. <t>K</t> <t>IL-33</t> (green) and CD31 (red) co-staining in human IPF vs normal, quantification is shown at right (n = 10). L IL-33 (green) and CD31 (red) co-staining in Yoda1- or GsMTx4-treated fibrotic mouse lungs, quantification is shown at right (n = 3). M IL-33 (green) and CD31 (red)co-staining in Piezo1 WT and Piezo1 ΔEC BLM lungs, quantification is shown at right (n = 3). Error bars represent mean ± SEM, H, K, J, M: two-tailed unpaired t-test; L: one-way ANOVA followed Tukey’s multiple comparwasons test, All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Human Il 33 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+human+il+33/Human+IL-33+Quantikine+ELISA+Kit/pmc13004862-358-11-16
Average 94 stars, based on 78 article reviews

human il 33 duoset elisa kit - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis"

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

Journal: Nature Communications

doi: 10.1038/s41467-026-70193-w

Figure Legend Snippet: A CellChat analysis of human lung snRNA-seq data. B Venn of 47 shared DEGs from four datasets ( GSE159354 , GSE132771 , GSE128033 , GSE122960 ). C Heatmap of 47-gene expression across cell types. D Correlation analysis (Pearson Correlation) of expression of IGFBP5, IL33, MGP, SPRY1, ACKR1 and mechanical stress scores in ECs. E Correlation analysis (Pearson Correlation) of expression of PIEZO1 and IGFBP5, IL33, MGP, SPRY1, ACKR1 in ECs. F Human snRNA-seq UMAP and violin of IL33 distribution and subpopulations. G Bar chart showed expression of IL33 (mean ± SEM) in IPF (n = 7966 cells) vs control (n = 4174 cells). P value was calculated using the two-tailed unpaired t-test. H Violin of IL33 in PIEZO1 - vs PIEZO1 + ECs. I Bulk-RNA-seq of saline and BLM (7 days and 14 days) induced mice (n = 3 per group). Gene expression levels were Z-score normalized. P value was calculated using the two-tailed unpaired t-test. J qPCR of Il33 in BLM mouse lung ECs. K IL-33 (green) and CD31 (red) co-staining in human IPF vs normal, quantification is shown at right (n = 10). L IL-33 (green) and CD31 (red) co-staining in Yoda1- or GsMTx4-treated fibrotic mouse lungs, quantification is shown at right (n = 3). M IL-33 (green) and CD31 (red)co-staining in Piezo1 WT and Piezo1 ΔEC BLM lungs, quantification is shown at right (n = 3). Error bars represent mean ± SEM, H, K, J, M: two-tailed unpaired t-test; L: one-way ANOVA followed Tukey’s multiple comparwasons test, All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Techniques Used: Gene Expression, Expressing, Control, Two Tailed Test, RNA Sequencing, Saline, Staining

A Schematic of BLM-induced fibrosis in Il33 WT vs Il33 ΔEC mice. B Il33 mRNA (n = 4) and IL-33 protein in lung ECs (n = 3). C Representative Masson/PSR images and quantification (n = 5). D αSMA immunofluorescence and quantification (n = 4). E Lung hydroxyproline content (n = 5). F Schematic: EC-specific Il33 overexpression via AAV-Tie1 in Piezo1 WT and Piezo1 ΔEC mice; 4-week pretreatment, BLM at week 4, sacrifice at week 7. G IL-33 expression in ECs, immune and epithelial cells after AAV-OE- Il33 (n = 3). H Masson/PSR images and quantification (n = 5). I αSMA immunofluorescence and quantification (n = 4). J Hydroxyproline content (n = 5). Error bars: mean ± SEM; B,G: two-tailed unpaired t-test; C,D-E,I: two-way ANOVA followed Tukey’s multiple comparisons test; H,J: three-way ANOVA followed Tukey’s multiple comparisons test. All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Figure Legend Snippet: A Schematic of BLM-induced fibrosis in Il33 WT vs Il33 ΔEC mice. B Il33 mRNA (n = 4) and IL-33 protein in lung ECs (n = 3). C Representative Masson/PSR images and quantification (n = 5). D αSMA immunofluorescence and quantification (n = 4). E Lung hydroxyproline content (n = 5). F Schematic: EC-specific Il33 overexpression via AAV-Tie1 in Piezo1 WT and Piezo1 ΔEC mice; 4-week pretreatment, BLM at week 4, sacrifice at week 7. G IL-33 expression in ECs, immune and epithelial cells after AAV-OE- Il33 (n = 3). H Masson/PSR images and quantification (n = 5). I αSMA immunofluorescence and quantification (n = 4). J Hydroxyproline content (n = 5). Error bars: mean ± SEM; B,G: two-tailed unpaired t-test; C,D-E,I: two-way ANOVA followed Tukey’s multiple comparisons test; H,J: three-way ANOVA followed Tukey’s multiple comparisons test. All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Techniques Used: Immunofluorescence, Over Expression, Expressing, Two Tailed Test

HUVECs under 20% stretch for 0,6,24 h or 2,12,25 kPa substrates; IL-33 secretion ( n = 3) A and mRNA (n = 3) B . C PIEZO1 knock-down with sh PIEZO1 ( n = 3). HUVECs were transduced with sh PIEZO1 for 48 h, cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch before measuring IL-33 secretion ( n = 3) D and mRNA ( n = 3) E . HUVECs were cultured on 2, 12, 25 kPa substrates for 24 h or stretched (20%) for 0, 6, 24 h, followed by measurement of calpain activity F and CAPN2 protein G ( n = 3). HUVECs (sh PIEZO1 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, followed by measurement of calpain activity ( n = 3) H and CAPN2 protein levels ( n = 3) I . J CAPN2 knock-down with shCAPN2 ( n = 3). K HUVECs (sh CAPN2 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, IL-33 secretion(left) and mRNA(right) ( n = 3). L Model for paracrine IL-33 regulation. M Cistrome: STAT3 predicted to drive IL-33 regulation. N intersection of top 100 motifs in BLM-treated mouse ECs with top 10 TFs. O The protein levels of P-STAT3 and STAT3 in HUVECs treated with 20% stretch for 0 and 6 hours ( n = 3). P The protein levels of P-STAT3 and STAT3 in HUVECs (sh PIEZO1 or sh CAPN2 ) treated with 20% stretch for 6 h ( n = 3). Q STAT3 knock-down with sh STAT3 ( n = 3). R HUVECs (sh STAT3 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch; IL-33 secretion(left) and mRNA(right) ( n = 3). Error bars: mean ± SEM; C , G , J , O – Q : two-tailed unpaired t-test; A , B , F : one-way ANOVA followed Šídák’s multiple comparisons test, D – E , H – I , K , R : two-way ANOVA Tukey’s multiple comparisons test. All n denote biologically independent samples. Source data are provided as a Source Data file.

Figure Legend Snippet: HUVECs under 20% stretch for 0,6,24 h or 2,12,25 kPa substrates; IL-33 secretion ( n = 3) A and mRNA (n = 3) B . C PIEZO1 knock-down with sh PIEZO1 ( n = 3). HUVECs were transduced with sh PIEZO1 for 48 h, cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch before measuring IL-33 secretion ( n = 3) D and mRNA ( n = 3) E . HUVECs were cultured on 2, 12, 25 kPa substrates for 24 h or stretched (20%) for 0, 6, 24 h, followed by measurement of calpain activity F and CAPN2 protein G ( n = 3). HUVECs (sh PIEZO1 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, followed by measurement of calpain activity ( n = 3) H and CAPN2 protein levels ( n = 3) I . J CAPN2 knock-down with shCAPN2 ( n = 3). K HUVECs (sh CAPN2 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, IL-33 secretion(left) and mRNA(right) ( n = 3). L Model for paracrine IL-33 regulation. M Cistrome: STAT3 predicted to drive IL-33 regulation. N intersection of top 100 motifs in BLM-treated mouse ECs with top 10 TFs. O The protein levels of P-STAT3 and STAT3 in HUVECs treated with 20% stretch for 0 and 6 hours ( n = 3). P The protein levels of P-STAT3 and STAT3 in HUVECs (sh PIEZO1 or sh CAPN2 ) treated with 20% stretch for 6 h ( n = 3). Q STAT3 knock-down with sh STAT3 ( n = 3). R HUVECs (sh STAT3 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch; IL-33 secretion(left) and mRNA(right) ( n = 3). Error bars: mean ± SEM; C , G , J , O – Q : two-tailed unpaired t-test; A , B , F : one-way ANOVA followed Šídák’s multiple comparisons test, D – E , H – I , K , R : two-way ANOVA Tukey’s multiple comparisons test. All n denote biologically independent samples. Source data are provided as a Source Data file.

Techniques Used: Knockdown, Transduction, Cell Culture, Activity Assay, Two Tailed Test

Image Search Results

a The experimental design. BMDMs treated by IL-33 (20 ng/mL) for 24 h before additional 24 h fresh medium incubation to prepare CMs. b Representative images and quantification of EdU+ VSMCs co-cultured with the indicated CMs. Scale bar, 100 μm. c Analysis of the putative ligand-receptor communications between arterial macrophages and VSMCs based on the scRNA-seq data of D0 and D7 using CellChat. d Volcano plot depicting the upregulated and downregulated genes in macrophages at D0 and D7. e Violin plots showing Spp1 gene expression of the 7 immune cell populations based on scRNA-seq data. f (Left) Representative flow cytometry gating for quantifying OPN-expressing macrophages and neutrophils at D7. (Right) OPN+ cell percentages and OPN signal intensities in macrophages and neutrophils at D7. n = 6 mice per group. g Representative images of immunofluorescence staining of MAC2 and OPN using the artery sections from mice at D7. Scale bars, 50 μm. h mRNA levels of Spp1 in the artery from mice at D0 ( n = 4), D2 ( n = 8), D4 ( n = 8), D7 ( n = 8) and D14 (n = 4). i Plasma OPN protein levels in patients with or without in-stent restenosis (ISR, n = 25; Non-ISR, n = 25). j mRNA levels of Spp1 of the artery from Control ( n = 7) and Pb- Il33 KO ( n = 8) at D7. k mRNA levels of Spp1 in the artery from Control ( n = 10) and Mac- Il1rl1 KO ( n = 7) at D7. l mRNA levels of Spp1 in IL-33-treated (20 ng/mL) BMDMs for 24 h. m BMDMs electro-transfected with si Spp1 followed by IL-33 (20 ng/mL) treatment to prepare CMs. n mRNA levels of the cell proliferation-related genes in indicated CM-exposed VSMCs. o Representative H&E staining images and Intima/Media ratios and lumen diameter/aspect ratios in the artery sections at D28. Mice post-FAI were administrated with the hydrogel containing si Spp1 or si NC locally at the injured sites. n = 6 mice for each group. Scale bars, 50 μm. Data are all shown as the mean ± s.e.m. ** p < 0.01 or *** p < 0.001 by unpaired two-tailed Student’s t -test ( f , i , j , k , n , o ), one-way ( b , h ) or two-way ANOVA ( l ). Source data and statistic information are provided as Source data files. Figure 7a,c, m were created in BioRender. Shan, B. (2025) https://BioRender.com/x16x8wd .

Journal: Nature Communications

Article Title: Perivascular mesenchymal cells instruct ST2+ reparative macrophages to promote endovascular injury-induced neointimal hyperplasia in mice

doi: 10.1038/s41467-026-68587-x

Figure Lengend Snippet: a The experimental design. BMDMs treated by IL-33 (20 ng/mL) for 24 h before additional 24 h fresh medium incubation to prepare CMs. b Representative images and quantification of EdU+ VSMCs co-cultured with the indicated CMs. Scale bar, 100 μm. c Analysis of the putative ligand-receptor communications between arterial macrophages and VSMCs based on the scRNA-seq data of D0 and D7 using CellChat. d Volcano plot depicting the upregulated and downregulated genes in macrophages at D0 and D7. e Violin plots showing Spp1 gene expression of the 7 immune cell populations based on scRNA-seq data. f (Left) Representative flow cytometry gating for quantifying OPN-expressing macrophages and neutrophils at D7. (Right) OPN+ cell percentages and OPN signal intensities in macrophages and neutrophils at D7. n = 6 mice per group. g Representative images of immunofluorescence staining of MAC2 and OPN using the artery sections from mice at D7. Scale bars, 50 μm. h mRNA levels of Spp1 in the artery from mice at D0 ( n = 4), D2 ( n = 8), D4 ( n = 8), D7 ( n = 8) and D14 (n = 4). i Plasma OPN protein levels in patients with or without in-stent restenosis (ISR, n = 25; Non-ISR, n = 25). j mRNA levels of Spp1 of the artery from Control ( n = 7) and Pb- Il33 KO ( n = 8) at D7. k mRNA levels of Spp1 in the artery from Control ( n = 10) and Mac- Il1rl1 KO ( n = 7) at D7. l mRNA levels of Spp1 in IL-33-treated (20 ng/mL) BMDMs for 24 h. m BMDMs electro-transfected with si Spp1 followed by IL-33 (20 ng/mL) treatment to prepare CMs. n mRNA levels of the cell proliferation-related genes in indicated CM-exposed VSMCs. o Representative H&E staining images and Intima/Media ratios and lumen diameter/aspect ratios in the artery sections at D28. Mice post-FAI were administrated with the hydrogel containing si Spp1 or si NC locally at the injured sites. n = 6 mice for each group. Scale bars, 50 μm. Data are all shown as the mean ± s.e.m. ** p < 0.01 or *** p < 0.001 by unpaired two-tailed Student’s t -test ( f , i , j , k , n , o ), one-way ( b , h ) or two-way ANOVA ( l ). Source data and statistic information are provided as Source data files. Figure 7a,c, m were created in BioRender. Shan, B. (2025) https://BioRender.com/x16x8wd .

Article Snippet: Plasma IL-33 and OPN protein levels were determined by a commercially available human IL33 ELISA Kit (#EK133, Multi sciences) and human OPN ELISA Kit (#EK1135, Multi Sciences) according to the manufacturer’s instructions.

Techniques: Incubation, Cell Culture, Gene Expression, Flow Cytometry, Expressing, Immunofluorescence, Staining, Clinical Proteomics, Control, Transfection, Two Tailed Test

Journal: Nature Communications

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

doi: 10.1038/s41467-026-70193-w

Figure Lengend Snippet: A CellChat analysis of human lung snRNA-seq data. B Venn of 47 shared DEGs from four datasets ( GSE159354 , GSE132771 , GSE128033 , GSE122960 ). C Heatmap of 47-gene expression across cell types. D Correlation analysis (Pearson Correlation) of expression of IGFBP5, IL33, MGP, SPRY1, ACKR1 and mechanical stress scores in ECs. E Correlation analysis (Pearson Correlation) of expression of PIEZO1 and IGFBP5, IL33, MGP, SPRY1, ACKR1 in ECs. F Human snRNA-seq UMAP and violin of IL33 distribution and subpopulations. G Bar chart showed expression of IL33 (mean ± SEM) in IPF (n = 7966 cells) vs control (n = 4174 cells). P value was calculated using the two-tailed unpaired t-test. H Violin of IL33 in PIEZO1 - vs PIEZO1 + ECs. I Bulk-RNA-seq of saline and BLM (7 days and 14 days) induced mice (n = 3 per group). Gene expression levels were Z-score normalized. P value was calculated using the two-tailed unpaired t-test. J qPCR of Il33 in BLM mouse lung ECs. K IL-33 (green) and CD31 (red) co-staining in human IPF vs normal, quantification is shown at right (n = 10). L IL-33 (green) and CD31 (red) co-staining in Yoda1- or GsMTx4-treated fibrotic mouse lungs, quantification is shown at right (n = 3). M IL-33 (green) and CD31 (red)co-staining in Piezo1 WT and Piezo1 ΔEC BLM lungs, quantification is shown at right (n = 3). Error bars represent mean ± SEM, H, K, J, M: two-tailed unpaired t-test; L: one-way ANOVA followed Tukey’s multiple comparwasons test, All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions.

Techniques: Gene Expression, Expressing, Control, Two Tailed Test, RNA Sequencing, Saline, Staining

Journal: Nature Communications

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

doi: 10.1038/s41467-026-70193-w

Figure Lengend Snippet: A Schematic of BLM-induced fibrosis in Il33 WT vs Il33 ΔEC mice. B Il33 mRNA (n = 4) and IL-33 protein in lung ECs (n = 3). C Representative Masson/PSR images and quantification (n = 5). D αSMA immunofluorescence and quantification (n = 4). E Lung hydroxyproline content (n = 5). F Schematic: EC-specific Il33 overexpression via AAV-Tie1 in Piezo1 WT and Piezo1 ΔEC mice; 4-week pretreatment, BLM at week 4, sacrifice at week 7. G IL-33 expression in ECs, immune and epithelial cells after AAV-OE- Il33 (n = 3). H Masson/PSR images and quantification (n = 5). I αSMA immunofluorescence and quantification (n = 4). J Hydroxyproline content (n = 5). Error bars: mean ± SEM; B,G: two-tailed unpaired t-test; C,D-E,I: two-way ANOVA followed Tukey’s multiple comparisons test; H,J: three-way ANOVA followed Tukey’s multiple comparisons test. All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions.

Techniques: Immunofluorescence, Over Expression, Expressing, Two Tailed Test

Journal: Nature Communications

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

doi: 10.1038/s41467-026-70193-w

Figure Lengend Snippet: HUVECs under 20% stretch for 0,6,24 h or 2,12,25 kPa substrates; IL-33 secretion ( n = 3) A and mRNA (n = 3) B . C PIEZO1 knock-down with sh PIEZO1 ( n = 3). HUVECs were transduced with sh PIEZO1 for 48 h, cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch before measuring IL-33 secretion ( n = 3) D and mRNA ( n = 3) E . HUVECs were cultured on 2, 12, 25 kPa substrates for 24 h or stretched (20%) for 0, 6, 24 h, followed by measurement of calpain activity F and CAPN2 protein G ( n = 3). HUVECs (sh PIEZO1 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, followed by measurement of calpain activity ( n = 3) H and CAPN2 protein levels ( n = 3) I . J CAPN2 knock-down with shCAPN2 ( n = 3). K HUVECs (sh CAPN2 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, IL-33 secretion(left) and mRNA(right) ( n = 3). L Model for paracrine IL-33 regulation. M Cistrome: STAT3 predicted to drive IL-33 regulation. N intersection of top 100 motifs in BLM-treated mouse ECs with top 10 TFs. O The protein levels of P-STAT3 and STAT3 in HUVECs treated with 20% stretch for 0 and 6 hours ( n = 3). P The protein levels of P-STAT3 and STAT3 in HUVECs (sh PIEZO1 or sh CAPN2 ) treated with 20% stretch for 6 h ( n = 3). Q STAT3 knock-down with sh STAT3 ( n = 3). R HUVECs (sh STAT3 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch; IL-33 secretion(left) and mRNA(right) ( n = 3). Error bars: mean ± SEM; C , G , J , O – Q : two-tailed unpaired t-test; A , B , F : one-way ANOVA followed Šídák’s multiple comparisons test, D – E , H – I , K , R : two-way ANOVA Tukey’s multiple comparisons test. All n denote biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions.

Techniques: Knockdown, Transduction, Cell Culture, Activity Assay, Two Tailed Test

Effect of obesity and sex on the circulating levels of interleukin (IL)-33 and soluble suppression of tumorigenicity 2 (sST2). Fasting serum concentrations of ( A ) IL-33, ( C ) sST2 and ( E ) IL-33/sST2 in normal-weight (NW-NG) volunteers ( n = 22), patients with obesity with normoglycemia (OB-NG) ( n = 24) and with obesity-associated type 2 diabetes (OB-T2D) ( n = 27). Circulating concentrations of ( B ) IL-33, ( D ) sST2 and ( F ) IL-33/sST2 in males ( n = 26) and females ( n = 47) volunteers. Data are mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests or by unpaired two-tailed Student’s t test as appropriate. * P < 0.05, ** P < 0.01 and *** P < 0.001

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: Effect of obesity and sex on the circulating levels of interleukin (IL)-33 and soluble suppression of tumorigenicity 2 (sST2). Fasting serum concentrations of ( A ) IL-33, ( C ) sST2 and ( E ) IL-33/sST2 in normal-weight (NW-NG) volunteers ( n = 22), patients with obesity with normoglycemia (OB-NG) ( n = 24) and with obesity-associated type 2 diabetes (OB-T2D) ( n = 27). Circulating concentrations of ( B ) IL-33, ( D ) sST2 and ( F ) IL-33/sST2 in males ( n = 26) and females ( n = 47) volunteers. Data are mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests or by unpaired two-tailed Student’s t test as appropriate. * P < 0.05, ** P < 0.01 and *** P < 0.001

Article Snippet: Circulating concentrations of IL-33 (D3300B) and sST2 (DST200) were quantified using commercial ELISA kits (R&D Systems, Minneapolis, MN) according to manufacturer’s instructions.

Techniques: Two Tailed Test

Gene and protein expression levels of IL-33 in visceral adipose tissue (VAT). A Bar graphs show the gene expression levels of IL33 in VAT samples from volunteers with normal weight (NW-NG, n = 8) and people with obesity and normoglycemia (OB-NG, n = 13) and with obesity-associated type 2 diabetes (OB-T2D, n = 16). B Protein expression levels of IL-33 in VAT from volunteers with normal weight (NW-NG, n = 4) and people with obesity and normoglycemia (OB-NG, n = 13) and with obesity-associated type 2 diabetes (OB-T2D, n = 10). C Scatter plots show the correlations between mRNA expression and protein levels of IL-33 as well as their associations with body fat percentage (BF). Correlation coefficients (r) and corresponding P values were calculated using Pearson’s correlation test. D Representative immunostaining for IL-33 in VAT samples from people with OB-NG and with OB-T2D (magnification 200x). E Comparison of gene expression levels of IL33 with IL1A , IL1B , IL4 , IL6 , IL8 , IL10 , IL13 , IL18 and IL32 in VAT obtained from people with obesity. Values are the mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests or by unpaired two-tailed Student’s t test as appropriate. * P < 0.05, and ** P < 0.01

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: Gene and protein expression levels of IL-33 in visceral adipose tissue (VAT). A Bar graphs show the gene expression levels of IL33 in VAT samples from volunteers with normal weight (NW-NG, n = 8) and people with obesity and normoglycemia (OB-NG, n = 13) and with obesity-associated type 2 diabetes (OB-T2D, n = 16). B Protein expression levels of IL-33 in VAT from volunteers with normal weight (NW-NG, n = 4) and people with obesity and normoglycemia (OB-NG, n = 13) and with obesity-associated type 2 diabetes (OB-T2D, n = 10). C Scatter plots show the correlations between mRNA expression and protein levels of IL-33 as well as their associations with body fat percentage (BF). Correlation coefficients (r) and corresponding P values were calculated using Pearson’s correlation test. D Representative immunostaining for IL-33 in VAT samples from people with OB-NG and with OB-T2D (magnification 200x). E Comparison of gene expression levels of IL33 with IL1A , IL1B , IL4 , IL6 , IL8 , IL10 , IL13 , IL18 and IL32 in VAT obtained from people with obesity. Values are the mean ± SEM. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests or by unpaired two-tailed Student’s t test as appropriate. * P < 0.05, and ** P < 0.01

Techniques: Expressing, Gene Expression, Immunostaining, Comparison, Two Tailed Test

Gene expression levels of inflammation-related factors in human visceral adipocytes treated with different concentrations of ( A ) IL-33 and ( B ) LPS for 3 h followed by IL-33 for another 24 h. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. ** P < 0.01. ADIPOQ, adiponectin; IL , interleukin; ITLN1 , omentin; LPS , lipopolysaccharide; TNF , tumor necrosis factor α

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: Gene expression levels of inflammation-related factors in human visceral adipocytes treated with different concentrations of ( A ) IL-33 and ( B ) LPS for 3 h followed by IL-33 for another 24 h. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. ** P < 0.01. ADIPOQ, adiponectin; IL , interleukin; ITLN1 , omentin; LPS , lipopolysaccharide; TNF , tumor necrosis factor α

Techniques: Gene Expression

A Effects of adipocyte-conditioned media (ACM) in monocytes-derived macrophages. Bar graphs show the effect of ACM (20 and 40%) from subjects with obesity incubated for 24 h on the transcript levels of IL33 . Impact of IL-33 on the expression levels of inflammation-related factors in monocytes-derived macrophages primed with ( B ) ACM and ( C ) LPS. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. * P < 0.05 and ** P < 0.01. CCL2 , monocyte chemoattractant protein-1; IL , interleukin; LPS , lipopolysaccharide; TNF , tumor necrosis factor-α

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: A Effects of adipocyte-conditioned media (ACM) in monocytes-derived macrophages. Bar graphs show the effect of ACM (20 and 40%) from subjects with obesity incubated for 24 h on the transcript levels of IL33 . Impact of IL-33 on the expression levels of inflammation-related factors in monocytes-derived macrophages primed with ( B ) ACM and ( C ) LPS. Values are the mean ± SEM ( n = 6 per group). Differences between groups were analyzed by one-way ANOVA followed by Tukey’s tests. * P < 0.05 and ** P < 0.01. CCL2 , monocyte chemoattractant protein-1; IL , interleukin; LPS , lipopolysaccharide; TNF , tumor necrosis factor-α

Techniques: Derivative Assay, Incubation, Expressing

$The increased circulating levels of IL-33 in obesity together the decrease in its soluble decoy receptor sST2 may be potentially enhancing IL-33 bioactivity. In addition, gene and protein expression levels of IL-33 in visceral adipose tissue (VAT) are upregulated in obesity in both adipocytes and stromal vascular fraction cells. IL-33 modulates adipocyte function by upregulating both anti-inflammatory mediators ( ADIPOQ , ITLN1 and IL13 ) and pro-inflammatory cytokines ( TNF , IL8 ). Under inflammatory conditions, such as LPS stimulation, IL-33 attenuates excessive pro-inflammatory responses and promotes protective adipokine expression in visceral adipocytes. In THP-1-derived macrophages, adipocyte-derived factors suppress IL33 expression, whereas IL-33 attenuates macrophage pro-inflammatory activation and enhances IL4 expression only in the context of adipocyte-conditioned medium. Together, these findings suggest the dual and context-dependent nature of IL-33 in obesity, acting as a compensatory mediator to maintain tissue homeostasis while potentially contributing to chronic low-grade inflammation when elevated chronically. ADIPOQ, adiponectin; IL , interleukin; ITLN1 , omentin; TNF , tumor necrosis factor α$

Journal: Molecular Medicine

Article Title: Dual and context-dependent role of the interleukin-33/soluble suppression of tumorigenicity 2 axis in obesity and adipose tissue inflammation

doi: 10.1186/s10020-026-01454-z

Figure Lengend Snippet: The increased circulating levels of IL-33 in obesity together the decrease in its soluble decoy receptor sST2 may be potentially enhancing IL-33 bioactivity. In addition, gene and protein expression levels of IL-33 in visceral adipose tissue (VAT) are upregulated in obesity in both adipocytes and stromal vascular fraction cells. IL-33 modulates adipocyte function by upregulating both anti-inflammatory mediators ( ADIPOQ , ITLN1 and IL13 ) and pro-inflammatory cytokines ( TNF , IL8 ). Under inflammatory conditions, such as LPS stimulation, IL-33 attenuates excessive pro-inflammatory responses and promotes protective adipokine expression in visceral adipocytes. In THP-1-derived macrophages, adipocyte-derived factors suppress IL33 expression, whereas IL-33 attenuates macrophage pro-inflammatory activation and enhances IL4 expression only in the context of adipocyte-conditioned medium. Together, these findings suggest the dual and context-dependent nature of IL-33 in obesity, acting as a compensatory mediator to maintain tissue homeostasis while potentially contributing to chronic low-grade inflammation when elevated chronically. ADIPOQ, adiponectin; IL , interleukin; ITLN1 , omentin; TNF , tumor necrosis factor α

Techniques: Expressing, Derivative Assay, Activation Assay

The ability to negate S. aureus -induced cytokine secretion from keratinocytes is a strain specific effect of M. luteus CFCS. (A, B) NHEK were treated with FSA or co-treated with FSA and skin bacterial CFCS for 24 h before quantifying IL-33 and TSLP in cell culture medium using ELISA. Stimulation of NHEK with FSA caused an increase in IL-33 and TSLP release. (B) Co-treatment with skin isolated M. luteus FAML CFCS negated FSA-induced release of IL-33 and TSLP. (C) Co-treatment with the M. luteus type strain NCTC 2665 had no effect on FSA-induced IL-33 and TSLP release. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

Journal: Frontiers in Immunology

Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

doi: 10.3389/fimmu.2026.1711723

Figure Lengend Snippet: The ability to negate S. aureus -induced cytokine secretion from keratinocytes is a strain specific effect of M. luteus CFCS. (A, B) NHEK were treated with FSA or co-treated with FSA and skin bacterial CFCS for 24 h before quantifying IL-33 and TSLP in cell culture medium using ELISA. Stimulation of NHEK with FSA caused an increase in IL-33 and TSLP release. (B) Co-treatment with skin isolated M. luteus FAML CFCS negated FSA-induced release of IL-33 and TSLP. (C) Co-treatment with the M. luteus type strain NCTC 2665 had no effect on FSA-induced IL-33 and TSLP release. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

Article Snippet: To quantify IL-33 and TSLP in NHEK conditioned medium, human IL-33 (R&D systems) and human TSLP (R&D Systems) DuoSet ELISA Kits were used according to manufacturer’s instructions.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Isolation

The efficacious molecule secreted by M. luteus FAML is a putative protein. M. luteus FAML was cultured for 1, 2, 4, 6 and 24 h before harvesting CFCS. NHEK were co-cultured with FSA and M. luteus FAML CFCS for 24 h before measuring (A) TSLP and (B) IL-33 in cell culture medium using ELISA. (C) M . luteus FAML CFCS collected at 24 h lost activity against FSA-induced IL-33 and TSLP release in NHEK after heat treatment (HT) to 85°C. (D) Proteins within M. luteus FAML CFCS were precipitated using acetone, then reconstituted in cell culture medium before testing for activity using the same model. Activity was retained within the protein precipitate (PP). Data are expressed as mean ± SEM (n≥4). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

Journal: Frontiers in Immunology

Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

doi: 10.3389/fimmu.2026.1711723

Figure Lengend Snippet: The efficacious molecule secreted by M. luteus FAML is a putative protein. M. luteus FAML was cultured for 1, 2, 4, 6 and 24 h before harvesting CFCS. NHEK were co-cultured with FSA and M. luteus FAML CFCS for 24 h before measuring (A) TSLP and (B) IL-33 in cell culture medium using ELISA. (C) M . luteus FAML CFCS collected at 24 h lost activity against FSA-induced IL-33 and TSLP release in NHEK after heat treatment (HT) to 85°C. (D) Proteins within M. luteus FAML CFCS were precipitated using acetone, then reconstituted in cell culture medium before testing for activity using the same model. Activity was retained within the protein precipitate (PP). Data are expressed as mean ± SEM (n≥4). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay

M. luteus FAML CFCS degrades IL-33 via serine protease activity. (A) Recombinant IL-33 or TSLP were treated with either M. luteus FAML CFCS or a medium control before quantifying the concentration of remaining IL-33 or TSLP within the sample using ELISA. Data are expressed as mean ± SEM (n=3). M. luteus FAML CFCS degraded IL-33 but not TSLP. P values determined by unpaired t test **P ≤ 0.01 compared with untreated IL-33 or TSLP control. (B) M. luteus FAML CFCS was pre-treated with the serine protease inhibitor AEBSF. Recombinant IL-33 was then treated with either AEBSF treated M. luteus FAML CFCS or untreated M. luteus FAML CFCS. Pre-treatment of M. luteus FAML CFCS with AEBSF inhibited the anti-IL-33 activity. Data are expressed as mean ± SEM (n=3). P values determined by one-way ANOVA **P ≤ 0.01 compared with untreated IL-33 control; ns, non-significant.

Journal: Frontiers in Immunology

Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

doi: 10.3389/fimmu.2026.1711723

Figure Lengend Snippet: M. luteus FAML CFCS degrades IL-33 via serine protease activity. (A) Recombinant IL-33 or TSLP were treated with either M. luteus FAML CFCS or a medium control before quantifying the concentration of remaining IL-33 or TSLP within the sample using ELISA. Data are expressed as mean ± SEM (n=3). M. luteus FAML CFCS degraded IL-33 but not TSLP. P values determined by unpaired t test **P ≤ 0.01 compared with untreated IL-33 or TSLP control. (B) M. luteus FAML CFCS was pre-treated with the serine protease inhibitor AEBSF. Recombinant IL-33 was then treated with either AEBSF treated M. luteus FAML CFCS or untreated M. luteus FAML CFCS. Pre-treatment of M. luteus FAML CFCS with AEBSF inhibited the anti-IL-33 activity. Data are expressed as mean ± SEM (n=3). P values determined by one-way ANOVA **P ≤ 0.01 compared with untreated IL-33 control; ns, non-significant.

Techniques: Activity Assay, Recombinant, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Protease Inhibitor

Recombinant PADP negates FSA-induced IL-33 and TSLP release from NHEK. NHEK were co-cultured with FSA and rPADP for 24 h before measuring IL-33 and TSLP in the cell culture medium using ELISA. The rPADP negated FSA-induced IL-33 and TSLP release in NHEK. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ****P ≤ 0.0001 compared with FSA treated NHEK.

Journal: Frontiers in Immunology

Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

doi: 10.3389/fimmu.2026.1711723

Figure Lengend Snippet: Recombinant PADP negates FSA-induced IL-33 and TSLP release from NHEK. NHEK were co-cultured with FSA and rPADP for 24 h before measuring IL-33 and TSLP in the cell culture medium using ELISA. The rPADP negated FSA-induced IL-33 and TSLP release in NHEK. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ****P ≤ 0.0001 compared with FSA treated NHEK.

Techniques: Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay

Review

Structured Review

Images

1) Product Images from "Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis"

Similar Products

Image Search Results